c26232t mutation in nsun7 gene and reduce sperm motility in asthenoteratospermic men

نویسندگان

nahid khosronezhad

abasalt hosseinzadeh colagar

syed golam ali jorsarayi

چکیده

reduced sperm quantity and motility are primary causes of infertility in men. before researchers showed that, nsun7 gene has roles in sperm motility of mouse, that creation defect in this gene is cause infertility. this gene in human located in chromosome 4, with 12 exons and a hot spot exon (exon7). our aim is study of the mutations of the exon7 in the normospermic and asthenoteratospermic men. for this, 30 semen samples including fertile and asthenoteratospermic men were collected from ivf center. semen analysis was performed according to who guidelines. a phenol-chloroform method was used for total dna extraction from sperm. the exons 7 amplified by forward primer sun7-f: 5’-gacaaatctcgaagtcttgctg; and reverse primer sun7-r: 5’-acatcctatttttgtgaaaagggt. the pcr products direct sequenced and analyzed for mutations. analyses of pcr direct sequences showed transition mutation (c26232t) in asthenoteratospermic men. this mutation doesn't see in fertile men. we probably that, low semen parameters of the asthenoteratospermic men can be close correlate with these mutations. thus, analyses of exon 7 direct sequence candidate as a one of diagnosis genetic markers of infertility.

برای دانلود باید عضویت طلایی داشته باشید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

C26232T Mutation in Nsun7 Gene and Reduce Sperm Motility in Asthenoteratospermic Men

Reduced sperm quantity and motility are primary causes of infertility in men. Before researchers showed that, Nsun7 gene has roles in sperm motility of mouse, that creation defect in this gene is cause infertility. This gene in human located in chromosome 4, with 12 exons and a hot spot exon (exon7). Our aim is study of the mutations of the exon7 in the normospermic and asthenoteratospermic men...

متن کامل

THE CORRELATION BETWEEN SPERM MORPHOLOGY AND MOTILITY IN FERTILE AND INFERTILE MEN

The swim-up procedure was used to collect a sperm fraction with improved motility and normal morphology from fertile and infertile semen samples. The untreated and swim-up fractions were analyzed with the Hamilton-Thorn Motility Analyser. Comparisons were made to see the effect of this sperm selection technique on sperm motility and morphology. They were then examined by transmission elect...

متن کامل

P-31: The Alteration of SpermatogenesisHas A Correlation with Sertoli Cell Mitochondrial Abnormal Morphology in Cytotoxicity of Testicular Tissue Mediatedwith Monosodium

Background: Male infertility has many causes, including genetic infertility. The NOP2/Sun domain family, member7 (Nsun7) gene, which encodes putative methyltransferase Nsun7, has a role in sperm motility. The aim of the present study was to investigate the effect of the T26248G polymorphism on Nsun7 protein function and its role in male infertility. Materials and Methods: Semen samples were col...

متن کامل

P-30: The Effect of The T26248G Polymorphism on Putative MethyltransferaseNsun7 Protein Function and Its Role in Male Infertility

Background: Male infertility has many causes, including genetic infertility. The NOP2/Sun domain family, member7 (Nsun7) gene, which encodes putative methyltransferase Nsun7, has a role in sperm motility. The aim of the present study was to investigate the effect of the T26248G polymorphism on Nsun7 protein function and its role in male infertility. Materials and Methods: Semen samples were col...

متن کامل

Sperm morphology, motility, and concentration in fertile and infertile men.

BACKGROUND Although semen analysis is routinely used to evaluate the male partner in infertile couples, sperm measurements that discriminate between fertile and infertile men are not well defined. METHODS We evaluated two semen specimens from each of the male partners in 765 infertile couples and 696 fertile couples at nine sites. The female partners in the infertile couples had normal result...

متن کامل

Effects of dietary selenium on sperm motility in healthy men.

A deficiency of dietary selenium leads to immotile, deformed sperm and infertility in rats, whereas supplementation of the diet with selenium compounds has been associated with both beneficial and deleterious effects on sperm function, depending on the chemical form of selenium. We conducted a randomized, controlled, and blinded intervention study on the effects of selenium in food on semen qua...

متن کامل

منابع من

با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید


عنوان ژورنال:
journal of genetic resources

ناشر: university of mazandaran

ISSN 2423-4257

دوره 1

شماره 1 2015

میزبانی شده توسط پلتفرم ابری doprax.com

copyright © 2015-2023